How to determine of Ractopamine in meat?

We know and we propose the solution!

Ractopamine is a drug that is used as a feed additive to promote leanness in animals raised for their meat. Pharmacologically, it is a beta-adrenoceptor agonist. It is the active ingredient in products known as Paylean for swine and Optaflexx for cattle, developed by Elanco Animal Health, a division of Eli Lilly and Company, for use in food animals for growth promotion.

Ractopamine has been banned in the European Union, Taiwan, mainland China and Russia.

As you know from the news: Rosselkhoznadzor on February 11, 2013. introduces prohibitions supply frozen meat and meat products from the United States. This is due to the fact that the American Veterinary Service does not observe the rules of ractopamine. Supply of chilled meat for that reason are prohibited from February 4. The use of ractopamine in Russia is prohibited. Rosselkhoznadzor notified the case management Safety and Inspection Service Food USDA.

How to determine of Ractopamine in meat?
We offer you the solution of this analytical task!

1. Extraction by ASE

Dionex ASEThe many steps involved in sample preparation can now be automated with ASE flow-through technology. Filtration and clean-up of solid samples can be achieved as part of the solvent extraction process in a single step. ASE offers a lower cost per sample than other techniques, reducing solvent consumption by up to 90%.

 

Dionex UltiMate 3000 RSLC Dual

 

 

 

2. Automated On-Line SPE-LC for HPLC Sample Preparation and Automation.

Solid-phase extraction (SPE) is a technique that is commonly used in laboratories to isolate the analytes of interest from complex matrices.

Example of analysis of pork:

Sample preparation: Extract 5,0 g pork chop with approx.. 50 ml of 4:1 acetonitrile : isopropanol using ASE extractor. Chill to precipitate fat, and filter.

In this example, we extracted pork with organic solvents in an Accelerated Solvent Extractor. Evaporation and reconstitution proved troublesome, so we avoided that by using solid-phase extraction (SPE).

SPE is most effective when it is orthogonal to the analytical separation, so we used cation exchange to retain the drugs, and washed away lipids and other interferences with organic solvent. The auxiliary valve then transferred the drugs to an Acclaim C30 column for RP-HPLC analysis.

The leaflet download here.

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  • 01 May 2017
  • Take part in School on the Ion chromatography!

    At 23-24th May  in operating time of the All-Russian conference "ANALYTICAL CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS" in Tuapse.

    The program of school for an ion chromatography includes the following primary subjects:

        Theoretical bases of an ion chromatography;
        Sorbents for an ion chromatography
        Devices of the ion chromatograph (detectors, design features, etc.);
        Proper laboratory practice (features of selection and preparation of samples for the analysis, methodical recommendations).
        Service of the chromatography equipment



    Participation: free, with obligatory advance registration

    Attention! The school on the Ion chromatography is carried out within the III ALL-RUSSIAN CONFERENCE "ANALYTICAL CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS" only for conferees.

    Concerning participation in a conference address to the organizing committee.

  • 28 February 2017
  • 15th International Exhibition of laboratory equipment and chemicals "Analytics Expo 2017"

    Abacus Analytical Systems GmbH will participate in the 15th International specialized exhibition of "Analyst Expo-2017" in ECC "Sokolniki" from April 11 to April 13, 2017. At an exhibition the analytical equipment of leading global manufacturers will be shown: HPLC chromatographs and the Ion chromatographs from Thermo-Dionex, parts of Thermo Fisher Scientific Inc. (USA), Elemental analyzers from Elementar (Germany), AAA analyzers and other analytical and biotechnological equipment.

    Visit the stand of our company at A211 in pavilion No. 4.

  • 18 September 2016
  • Seminar on the equipment Thermo for control of the environment and food on October 6 in Moscow

    Moscow | Thursday, October 6, 2016 | 10:00 - 17:00

     

    Please click to register on the seminar

     

    You will be able to learn in more detail about:

        New ion chromatography system Integrion
        The analysis of dioxine in food
        Use of an ion chromatography in the analysis of foodstuff
        Work with samples with the high content of a matrix by the ICP-MS method

  • 19 October 2015
  • Practical course on ion chromatography 16-20.11.2015
  • 05 August 2014
  • International conference "Disinfection By-products in drinking water"

    International conference "Disinfection By-products in drinking water"

    27th-29th October 2014
    Municipal Hall, Mulheim an der Ruhr, Germany

    This international conference will be organised by IWW Water Centre (DE), RSC (UK) and SCI (UK). A scientific poster session and an exhibition related to the topics of the conference will be included. It is expected that more than 200 attendees from all over Europe will take part. You will have the opportunity to talk to decision makers from water companies and regulators. The relevant scientific community will also be present. The programme comprises eleven keynote speakers and some twenty speakers from the call for papers.