How to determine of Ractopamine in meat?

We know and we propose the solution!

Ractopamine is a drug that is used as a feed additive to promote leanness in animals raised for their meat. Pharmacologically, it is a beta-adrenoceptor agonist. It is the active ingredient in products known as Paylean for swine and Optaflexx for cattle, developed by Elanco Animal Health, a division of Eli Lilly and Company, for use in food animals for growth promotion.

Ractopamine has been banned in the European Union, Taiwan, mainland China and Russia.

As you know from the news: Rosselkhoznadzor on February 11, 2013. introduces prohibitions supply frozen meat and meat products from the United States. This is due to the fact that the American Veterinary Service does not observe the rules of ractopamine. Supply of chilled meat for that reason are prohibited from February 4. The use of ractopamine in Russia is prohibited. Rosselkhoznadzor notified the case management Safety and Inspection Service Food USDA.

How to determine of Ractopamine in meat?
We offer you the solution of this analytical task!

1. Extraction by ASE

Dionex ASEThe many steps involved in sample preparation can now be automated with ASE flow-through technology. Filtration and clean-up of solid samples can be achieved as part of the solvent extraction process in a single step. ASE offers a lower cost per sample than other techniques, reducing solvent consumption by up to 90%.

 

Dionex UltiMate 3000 RSLC Dual

 

 

 

2. Automated On-Line SPE-LC for HPLC Sample Preparation and Automation.

Solid-phase extraction (SPE) is a technique that is commonly used in laboratories to isolate the analytes of interest from complex matrices.

Example of analysis of pork:

Sample preparation: Extract 5,0 g pork chop with approx.. 50 ml of 4:1 acetonitrile : isopropanol using ASE extractor. Chill to precipitate fat, and filter.

In this example, we extracted pork with organic solvents in an Accelerated Solvent Extractor. Evaporation and reconstitution proved troublesome, so we avoided that by using solid-phase extraction (SPE).

SPE is most effective when it is orthogonal to the analytical separation, so we used cation exchange to retain the drugs, and washed away lipids and other interferences with organic solvent. The auxiliary valve then transferred the drugs to an Acclaim C30 column for RP-HPLC analysis.

The leaflet download here.

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    "Abacus Analytical Systems" and the Thermo Fisher Scientific invite you to participate in a scientific and practical seminar "The latest analytical equipment for an Ion and Liquid Chromatography from the Thermo Scientific with its brand Dionex™ ". Seminar will take place in Moscow on May 16, 2019 in the conference center at hotel of "Maxima Zarya" (metro Okrujhnaya, Vladykino or Petrovsko- Razumovskaya). Lectures will be given by leading experts in the field of Ion and liquid chromatography (Dr. Joachim Weis, Roman Repas from Thermo Scientific and Elena Rybakova from Abacus Analytical Systems).

    Participation in a seminar - free. For participation it is necessary to be registered, write us to the address: lab@abacus-lab.ru

  • 11 March 2019
  • Practical course on ion chromatography 18-22 November 2019 in Moscow

    Nearest practical course on ion chromatography will take place in Moscow on the territory of Thechnical University (MISIS) 18-22th Novamber 2019.

    The program of course for an ion chromatography includes the following primary subjects:

        Theoretical bases of an ion chromatography;
        Sorbents for an ion chromatography
        Devices of the ion chromatograph (detectors, design features, etc.);
        Proper laboratory practice (features of selection and preparation of samples for the analysis, methodical recommendations).
        Service of the chromatography equipment

    Please contact us for future details!

  • 14 January 2019
  • 2019 is the 150th anniversary of the Periodic Table of Chemical Elements

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    1869 is considered as the year of discovery of the Periodic System by Dmitri Mendeleev. 2019 will be the 150th anniversary of the Periodic Table of Chemical Elements and has therefore been proclaimed the "International Year of the Periodic Table of Chemical Elements (IYPT2019)" by the United Nations General Assembly and UNESCO.

    Read more on web: https://www.iypt2019.org/

     

    Mendeleev 150: 4th International Conference on the Periodic Table endorsed by IUPAC (link to web-site of Conference)

    Sep 06-13, 2019, Saint Petersburg, Russia

  • 24 June 2018
  • Practical course on ion chromatography 19-23 November in Moscow

    Nearest practical course on ion chromatography will take place in Moscow on the territory of Thechnical University (MISIS).

    The program of course for an ion chromatography includes the following primary subjects:

        Theoretical bases of an ion chromatography;
        Sorbents for an ion chromatography
        Devices of the ion chromatograph (detectors, design features, etc.);
        Proper laboratory practice (features of selection and preparation of samples for the analysis, methodical recommendations).
        Service of the chromatography equipment

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  • Practical course on ion chromatography 19-23 November in Moscow (1)

    Nearest practical course on ion chromatography will take place in Moscow on the territory of Thechnical University (MISIS).

    The program of course for an ion chromatography includes the following primary subjects:

        Theoretical bases of an ion chromatography;
        Sorbents for an ion chromatography
        Devices of the ion chromatograph (detectors, design features, etc.);
        Proper laboratory practice (features of selection and preparation of samples for the analysis, methodical recommendations).
        Service of the chromatography equipment