Expect better instrument control from your chromatography
software. Chromeleon® controls over 300 different LC,
IC, and GC instruments and accessories from more than 30
manufacturers, with full bi-directional communication, extensive
command options, and detailed event tracking. You can share methods
between different instruments, optimize control panels for
different users, and even set conditional triggers in the software
to respond intelligently to changing conditions. No other software
makes it so easy to harmonize operations throughout your
Chromeleon provides full control of over 300 instruments from
more than 30 manufacturers. No other software offers such broad
Setting up instrument control is fast and easy with the
Chromeleon Control Program Wizard.
Control diverse instruments using a single unified command
Customize the user interface to suit your needs with
Chromeleon's unique control panel editor.
Get More Information from your Samples with Advanced
Data Analysis Capabilities
If your work is more challenging than routine chromatography,
you need more powerful analytical tools than the ones available
with most data systems. The capabilities of Chromeleon®
go well beyond the basics to help you through your toughest
Diode-Array Data Analysis
With the optional Chromeleon PDA license, you can acquire
full-spectrum data from selected diode-array detectors. Those data
can then be processed on any Chromeleon® client station
for 3-D data rendering, peak purity analysis, and compound
identification based on spectral library matches. All of these
capabilities are fully and seamlessly integrated into
Chromeleon®-you never feel like you're working with a
separate piece of software.
LC/MS and IC/MS
With the MS option, Chromeleon offers full control, data
acquisition, and data processing for the Thermo MSQ™ and
AQA™ mass spectrometers. You can check the mass spectrum
of an eluting peak in real time, compare mass spectra corresponding
to different chromatographic peaks-even overlay TIC and SIM
chromatograms with data from UV, fluorescence, and conductivity
detectors to confirm analyte identities and spot impurities.
Custom Sample Variables and Peak Variables
Access the full power of Chromeleon's built-in spreadsheet using
Chromeleon's optional Report Publisher. Add columns to the sequence
to store lot codes, expiration dates, sampling points, special
correction factors, and other variables that have a correspondence
of one per sample. Add columns to the peak tables to store
calibration standard descriptions, purity factors, stability
factors, and other variables that differ from compound to
Custom variables can be any of several data types (text,
integer, floating point value, date/time field, or "pick list"),
and once defined, are available throughout the software for
labeling, calculations, database queries, and reporting.
With Chromeleon's optional Report Publisher, you can access the
full power of Chromeleon's built-in spreadsheet. Create your own
formulas in standard Microsoft Excel syntax, using a library of
over 120 worksheet functions such as IF, AVERAGE, STDEV, and
LOOKUP. Use cross-references between worksheets to share calculated
values across multiple report sections, or to separate intermediate
calculations from the final presentation. And because the Report
Publisher also supports charting, you can present results
graphically for easier interpretation.
Software Development Kit
Want even more flexibility? Need a completely customized user
interface, or a solution that integrates Chromeleon with other
software products? The Chromeleon Software Development Kit (SDK)
gives you the tools you need to develop such custom solutions.
Using Visual Basic, C++, Delphi, or any other programming language
that supports the component object model (COM), you can read and
write Chromeleon data structures and system variables, and make
function calls to perform a wide variety of automation and data
processing tasks. With the Chromeleon SDK, the possibilities are
limited only by your imagination and programming resources.
Three-dimensional data analysis features are seamlessly
integrated into Chromeleon, so you never feel like you're working
with a separate software product.
With its friendly user interface and powerful analysis
capabilities, Chromeleon makes LC/MS practical for a broad range of
Define your own custom variables to store lot codes, expiration
dates, purity factors, and other data relevant to your specific
application. Use the variables anywhere you like in Chromeleon-in
calculations, reports, even peak labels.
With Chromeleon®, you can easily analyze your data
and present the results in the ways your customers want to see
them. The built-in spreadsheet works like Microsoft®
Excel, so it is both powerful and familiar. Chromeleon®
report workbooks can have as many worksheets as you like; each
worksheet can include result tables, chromatograms, calibration
plots, spectra, audit trails, and even custom equations and charts.
Every cell, table, and chart updates instantly if any of the source
data change, so you never have to worry about consistency. You get
the custom reports you need without the hassles and validation
issues of exporting to other software, so you save time, effort,
Chromeleon gives you the power and flexibility of an
Excel-compatible spreadsheet directly within the chromatography
software environment. Results are calculated dynamically, so they
are always up to date.
The on-screen results display gives you instant feedback as you
browse through chromatograms or make adjustments.
In the printer layout mode, you can see report pages exactly as
they will be printed.
With the Report Publisher option, you can perform custom
calculations by entering custom worksheet formulas in standard
Microsoft Excel syntax, and also include custom charts in your
The laws are strict and enforcement is getting tougher. A single
compliance violation could cost your company millions in fines and
lost revenues. You know you need to comply with the rules, but how
can you make your existing chromatography systems and operations
compliant? How can you cope with an increasing workload of analyses
while still ensuring that you're always ready to pass an audit?
You need a solution that meets compliance requirements and
increases your productivity. You need Chromeleon®.
Chromeleon® software is more than your best choice
for a chromatography workstation-it's also a complete multi-user
chromatography management solution that adapts to diverse needs
throughout your enterprise. You can start with a small installation
and scale it up at your own pace using the operating system and
database platforms that best suit your needs. Get the benefits of
centralized data storage and system administration without giving
up the flexibility of distributed workstations. Standardize or
customize user interfaces, methods, and reports as appropriate to
address different users and different applications. Integrate
Chromeleon® software with other software products to
further improve overall laboratory productivity and
Expand your Chromeleon® network easily and economically
by adding workstations and remote client stations as your needs
grow. The Chromeleon software's distributed client/server
architecture continues to deliver high performance as you scale up
Choose the platforms that fit your needs, and migrate with
ease. Chromeleon software works seamlessly with any
combination of PCs running Windows® 2000, Windows XP,
and/or Windows Vista, and with any combination of SQL Server,
Oracle®, or Microsoft® Access database
Administer your Chromeleon® systems from anywhere in
the network. You can configure instruments, set policies for
instrument and data access, manage users and groups, and administer
data warehouses-all with the convenience of remote access.
Put your mind at ease. Chromeleon® software keeps
working productively, even in situations that would paralyze
ordinary data systems. With its distributed client/server
architecture, intelligent instrument control, network failure
protection, and power failure recovery, Chromeleon®
software provides the high availability and fault tolerance you
need for optimum productivity.
Integrate Chromeleon® software seamlessly with other
software in your laboratory. Copy chromatograms, spectra, and other
graphics to office applications to create journal publications and
custom presentations. Import sample worklists from your LIMS,
and post results to it automatically. Export reports in popular
formats such as XLS and PDF, with every detail faithfully
Get the most out of your chromatography software with
enhancements and extensions that provide advanced automation
options. One example is AutoDilution which enables
Chromeleon® software to automatically dilute and
reinject samples that exceed a calibration range.
Challenging samples such as environmental and waste water often
contain compound concentrations that exceed the range defined by a
standard curve. Operators would need to identify these outliers,
dilute the samples, and often rerun the standards along with the
diluted samples. These time consuming steps have now been
eliminated with a software enhancement called AutoDilution.
The AutoDilution license enables Chromeleon to automatically
determine when compound concentrations exceed a set concentration,
perform a dilution of these samples, and rerun them along with
standards if needed.
The basic operation uses a smaller injection volume in the
Depending on the hardware setup, the following options are
- Smaller injection volume in the existing loop using partial
loop injections with the AS Autosampler.
- Vial-to-vial dilution performed with the AS Autosampler using
the Sample Prep module.
- Secondary loop fill and injection using additional valves like
the ones available for the AS Autosampler, AS-DV,
ICS-1100/1600/2100, and in the Automation Manager for the DC
Note: The AutoDilution option provides the same capabilities as
the Extended Fractionation License and will appear as "Extended
Fractionation Collection" in the license features list.
Distributed client/server: "server" software module manages all
real-time instrument control and data acquisition tasks; "client"
software module provides all user interface functions. Any number
of client instances can connect to any number of server instances.
Client and server modules can be used together on the same PC (a
"workstation"), or on separate PCs ("instrument controllers" and
Microsoft® Windows® XP, Vista or Windows
2000 (can be mixed in network installations).
- Fast retrieval of samples based on any combination of sample,
sequence, and result criteria.
- Compatible with Oracle® 8i, 9i, 10g, Microsoft SQL
Server 7, Microsoft SQL Server 2000, Microsoft SQL Server 2005, and
Microsoft Jet Engine 3/4 (Access MDB format).
Fast, easy generation of sequences using Sequence Wizard or LIMS
Worklist import. Unlimited number of samples per sequence;
unlimited number of sequences per batch.
- Up to six timebases (independent instrument systems) per
workstation or instrument controller.
- Up to two diode-array detectors per workstation.
- Full control for over 300 different instruments from more than
30 manufacturers through RS-232, IEEE-488, DX-LAN™, USB,
LAN, etc. Programmable triggers for conditional execution of
instrument control commands.
- Direct digital data acquisition from most Dionex detectors;
acquisition from other instruments via UCI Universal Chromatography
Interfaces at rates up to 100 Hz with 25-bit dynamic
- Up to 16 standard detector channels per time base
(chromatography system), more for photodiode array and mass
- Chromatogram run-times up to 999.999 min.
Peak Detection and Identification
- More than 25 time-programmable detection options.
- Easy manual manipulation of baselines.
- Component identification based on absolute or relative
retention time, with or without spectral matching.
- Up to 1024 sample components per method.
- Independently selectable identification parameters for each
- Unlimited number of component groups per method.
- External standard, internal standard, and internal/external
- Five sequence calibration mode options (fixed, total,
additional, group, and bracketed).
- Linear, quadratic, cubic, point-to-point, and exponential
- 1/x, 1/x², 1/y, 1/y² polynomial curve
- Choice of origin treatment (force, include, ignore).
- Quantitation of identified and unidentified components using
fixed or relative response factors.
- Standard Addition methodology support.
- Option to invert variables before curve fitting.
- Blank and Matrix Blank correction options.
- Independently selectable calibration options for each
- Manual enable/disable of individual calibration points.
- Instant updating of all results in response to any change (can
- Integrated Microsoft Excel-compatible spreadsheet provides
complete reporting flexibility with dynamic updating.
- Extensive set of report variable and report table options
facilitates generation of single-injection, calibration, summary,
system suitability, audit trail, fraction collection, and other
types of reports.
- User-defined sample and peak variables are fully
- Optional Report Publisher allows incorporation of charts and
user-defined formulas written in Excel syntax.
Optional Software Components
- PDA Control supports collection of full-spectrum UV data from
up to 2 photodiode-array detectors per workstation. (Peak purity
analysis, spectral library matching, and method optimization tools
can be used on any client station that has access to the acquired
- Mass Spectrometer Support for Finnigan AQA™ and
Finnigan MSQ™ (Thermo Electron Corp.) provides full
instrument control in ESI and APCI modes with positive and/or
negative ionization; supports full-scan and multiple SIM channels;
generates extracted ion chromatograms (post-run or real-time) and
TICs; and provides interlinked, interactive displays of mass
spectra and chromatograms from multiple channels. Supports advanced
applications like mass-directed fraction collection.
- Multiple Network Control permits instruments attached to a
workstation or instrument controller to be accessed and controlled
through remote clients, and provides network failure
- Report Publisher supports custom equations and charting.
- GLP Option provides user access control, electronic signature,
and modification history features.
- Virtual Column Separation Simulator models gradient and
isocratic IC separations and quickly finds optimum conditions for
various IC applications.
Security can be implemented at 3 levels: operating system,
database, and application. At the application level, over 100
chromatography-specific privileges can be separately controlled for
an unlimited number of Privilege Groups. Access to individual
instruments, data sources, and data folders can be controlled for
an unlimited number of Access Groups. Numerous administrator tools
are provided. (GLP Option required).
- Certificate of Software Validation provided with software
- AutoQ™ - a full complement of automated validation
tools including Software IQ, Software OQ, Instrument OQ, and
- Secure, computer-generated, time-stamped audit trails
automatically record details of system usage:
- Instrument Event Logs document all instrument and operator
actions related to data acquisition.
- Modification Histories document all changes made to control
programs, methods, data objects, etc. (GLP Option required).
- User Database Histories document user logins, login failures,
password changes, privilege changes, and electronic signature
events. (GLP Option required).
Complete, seamless implementation of electronic signatures,
compliant with requirements of 21 CFR Part 11 (GLP Option
Software Interfacing Capabilities
- Import of ASCII sample worklists.
- Direct import of raw data from Dionex PeakNet®,
Agilent ChemStation, Agilent LAS and ANDI formats.
- Export of results as text, native Microsoft Excel, and Portable
Document Format (PDF) files.
- Export of raw data in ANDI format (Types 1 and 2) and ASCII
- Direct interfacing to third-party applications through COM
model using optional Software Developer's Kit. Fileless interfacing
to LIMS using Labtronics LimsLinkCDS.
Virtual Column Separation Simulator
Discover the fastest, easiest, and most economical way to
optimize IC separations.
Why Optimize IC the Hard Way?
Seeking the best column and separation conditions for an IC
analysis used to be a gamble. You had to guess where to start and
which parameters to vary, never sure if you were headed in the
right direction. You had to spend hours generating chromatograms,
consuming samples and eluents while tying up your instrument and
putting wear and tear on your pump, autosampler, and column.
Fast, Risk-Free Optimization
Virtual Column eliminates the risks and hassles in optimizing IC
separations by modeling their behavior in software, using known
retention data and IC-specific retention algorithms. Specify the
analytes you want to separate, choose the Dionex column and eluent
system you want to model, and Virtual Column can find and display
the optimal separation, together with a resolution map indicating
the eluent concentration(s) required.
Easy Answers to Challenging
Want to shorten the run time, "balance" the separation of the
peaks, or resolve a pair of difficult peaks? Simply click a command
to find the conditions that will yield the fastest chromatogram
with acceptable resolution, or the best "spread" of the peaks, or
the best resolution of two specific peaks.
Solutions to Cover Your Application
Virtual Column models all of the most popular Dionex
anion-exchange and cation-exchange columns, using retention data
for hundreds of analytes - you don't spend any time "training" the
software. Use Virtual Column in Chromeleon's powerful Application
Wizard and generate the exact Control Program and Quantification
method for your newly developed separation.
Develop Gradient Separations as Easily as Isocratic
Gradient separations can greatly increase the capability and
performance of ion chromatography, but until recently, only experts
were willing to try them. Now with a Virtual Column Complete
license and Reagent-Free™ IC (RFIC™) systems,
everyone can take advantage of the power of gradient ion
chromatography. A few minutes invested into modeling your
application under gradient conditions could save you hundreds of
hours per year.
Optimize Separation of Your Specific
When sulfite is added to the "standard anions", it coelutes with
phosphate under standard separation conditions. In just a few
seconds, Virtual Column finds conditions that separate all analytes
with baseline resolution.
Speed Up Your Separations
Standard separation conditions for the AS15 column yield
excellent resolution of "standard anions" and thiosulfate, but
result in an analysis time of over 36 min.
In seconds, through a single menu command, Virtual Column finds
conditions that dramatically reduce analysis time, while
maintaining resolution above your specified minimum.
Isolate a Specific Peak of
Virtual Column quickly reveals that baseline resolution of this
set of analytes cannot be achieved with the CS12A
column-potentially saving hours or days of futile effort.
If the analytical need is to quantify a particular analyte,
Virtual Column quickly finds the conditions that maximize
resolution of the key analyte from other species.
Workstation or client station with Chromeleon Release 6.6 or
higher. Gradient simulations require Chromeleon Release 6.7 or
Include Virtual Column on the same purchase order that you use
to order the corresponding Chromeleon workstation or client
package. To add Virtual Column to an existing Chromeleon
workstation or client station, provide the serial number of that
station when placing your order. (To find the serial number of a
station, select "About" from the "Help" menu of the Chromeleon
Chromeleon 6.8 - Extension Pack Version 2.0
Get Results Immediately!
Dionex introduces version 2.0 of the Extension Pack for
Chromeleon® 6.8 software. This free pack contains
Sequence, Query, and Report templates that allow you to quickly and
easily perform and report common tests that otherwise can take
considerable amounts of time to process. Most tests involve
comparing the results to specifications. The templates do this for
you automatically and let you know immediately if your tests have
passed or failed.
Let Chromeleon software transform your laboratory and increase
your analytical efficiency. For more information, contact your
local Dionex representative.