On-line analysis in HPLC
Integral™ systems provide a versatile and adaptable approach to
on-line HPLC systems. An unsurpassed range of HPLC capabilities,
including UHPLC and biocompatible systems, can be combined with
configurable sampling and sample preparation systems and adaptable
off-the-shelf industrial enclosure options. Configure solutions for
the R&D lab, pilot plant, or production area, and migrate the
methods easily through process development steps. This allows you
to focus engineering resources on implementation, not on
- Integral sampling modules and enclosures can be configured with
conventional UltiMate® 3000 systems or Rapid Separation LC
(RSLC) UltiMate 3000 UHPLC systems, available in standard or
biocompatible configurations. Dual systems are available for
increased method flexibility and throughput.
- The Integral Migration Path™ approach to on-line implementation
means systems can be configured for bench-top process R&D
studies, pilot plant or dedicated plant floor installations.
- Versatile sampling and sample preparation capabilities provide
support for system multiplexing and core functions such as
dilutions, concentration, matrix elimination, and reagent
- Off-the-shelf enclosure options provide environmental
protection and industrial compliance, when and where you need
- Chromeleon® PA Process Analytical
software provides system control, status, reporting, and
connectivity with industrial control systems using industry
standard OPC protocol.
- Support for PAT systems is based on 25 years of experience in a
wide range of industries worldwide.
Typical analyzers are configured with one or more HPLC systems,
all analyzing the same sample in parallel. Each of these systems
performs a specific HPLC analysis. Fast analysis times can be
achieved with the UltiMate 3000 RSLC system.
An analyzer can be configured to handle from 1 to 21 sample
streams. Sample streams are analyzed in series with the order and
frequency determined by the user through software control.
Each system is configured with an appropriate analytical pump,
detector, accessories, and set of analysis columns. These
components are determined by the specific analysis being run on the
SP Sample Preparer
The Integral SP Sample Preparer provides versatile capabilities
for sample preparation prior to chromatographic analysis. The SP is
designed for continuous on-line monitoring applications. Standard
functions include sample and standard selection, preparation
(dilution, reagent addition, etc.), and loading. Pressure, flow,
and pH monitoring are all options. The SP can be utilized with
Dionex benchtop HPLC systems or with industrial systems configured
within AE and LE enclosures.
SS Stream Selector
The Integral SS Stream Selector provides a full-featured module
which automatically selects from up to 21 process streams for
sequential analysis. The SS can be configured to deliver samples to
up to four HPLC systems running in parallel. Sample streams can be
configured to run to waste or sample recovery, deadhead at the SS
valve, or recirculate back to the process.
Analyzer Enclosure (AE)
The Integral Analyzer Enclosure (AE) provides an adaptable
housing for a variety of HPLC system configurations. Stainless
steel enclosure construction provides compatibility with harsh
operating environments, as well as the value of a long-lasting
investment. The AE is designed for NEMA 12 / IP65 areas in its
basic configuration. Options are available for configuring for NEC
C1D2 / EU Zone 2 or NEC C1D1 / Zone 1 rated hazardous environments.
Optional air conditioning allows operation in a wider range of
The AE can be wall-mounted or installed on a LE Liquids
Enclosure for a free-standing or wheeled unit. The AE also houses
the PC-based controller, flat-panel display, and touch-mouse
device. Power input and automation connections are directed through
the top of the AE. Liquid and gas connections are directed through
the bottom and sides of the AE and LE.
LE Liquids Enclosure
The Integral LE Liquids Enclosure provides an optional base for
the AE as well as a means of isolating and protecting eluent,
reagent, and standards containers. It can also be used as an
expansion enclosure for additional LC modules. The LE is designed
to be NEMA 12 compliant, and can be configured for NEMA 4X / IEC
Automation and Control
Dionex-designed Chromeleon PA software provides configuration,
control, analyzer, and system status, results display and trending,
along with user-defined alarm actions and results reporting. All
instrumentation is software controlled. All raw data is stored in a
secure database with audit trail support. Multiple levels of
security are available for access determined by the system
administrator. For applications in the pharma/biopharma industry,
Chromeleon PA meets the regulatory requirements of CFR 21, Part
Process Analytical Market Solutions
Process Analytical systems from Dionex find applications in a
wide range of industries and environments, offering the confidence
of timely and trustworthy data, the advantages of knowledgeable
process control, and the value of profitable process quality.
Hundreds of Dionex on-line ICs and HPLCs are already in use
worldwide; from process development laboratories to remote
monitoring stations and manufacturing plant floors.
Use on-line HPLC systems for real-time determination of product
quality in purification processes or determining reaction endpoint
or impurity profiles in synthesis reactions. Use on-line
Reagent-Free™ IC (RFIC™) systems for
monitoring nutrient levels such as amino acids, organic acids, and
carbohydrates in fermentation reactors. Dionex offers a host of
separation capabilities that are adaptable for on-line
On-line RFIC systems are used to monitor corrosion by-products
in coolants and steam condensates, as well as make-up water
quality. Systems are used in the nuclear power industry at PWR and
BWR plants to monitor primary and secondary chemistries. Fossil
fuel power plants find utility for migration systems in sampling
labs, as well as on-line.
Process analytical RFIC systems are used to monitor ultrapure
water and process chemicals. Key applications include
part-per-trillion ion analysis in ultrapure water and determination
of trace contaminants in semiconductor grade acids, bases, and
Analyzing environmental samples at the source, on-line RFIC and
HPLC systems are used to determine naturally occurring species in
river and delta waters, organic waste intermediates in industrial
effluent processes, and toxics at hazardous materials remediation
On-line RFIC and HPLC systems are used to determine
concentrations of product in reactors and to monitor contaminants
in process and waste streams.
Food and Beverage
Use HPLC and RFIC systems for real-time determination of product
quality and product development processes. Process analytics can
save time and improve quality for wide range of processes and
products, including fermentation broths, purification steps, and
other processes used to produce items such as dairy products, soft
drinks, fruit juices, and bottled water.