How to determine of Ractopamine in meat?

We know and we propose the solution!

Ractopamine is a drug that is used as a feed additive to promote leanness in animals raised for their meat. Pharmacologically, it is a beta-adrenoceptor agonist. It is the active ingredient in products known as Paylean for swine and Optaflexx for cattle, developed by Elanco Animal Health, a division of Eli Lilly and Company, for use in food animals for growth promotion.

Ractopamine has been banned in the European Union, Taiwan, mainland China and Russia.

As you know from the news: Rosselkhoznadzor on February 11, 2013. introduces prohibitions supply frozen meat and meat products from the United States. This is due to the fact that the American Veterinary Service does not observe the rules of ractopamine. Supply of chilled meat for that reason are prohibited from February 4. The use of ractopamine in Russia is prohibited. Rosselkhoznadzor notified the case management Safety and Inspection Service Food USDA.

How to determine of Ractopamine in meat?
We offer you the solution of this analytical task!

1. Extraction by ASE

Dionex ASEThe many steps involved in sample preparation can now be automated with ASE flow-through technology. Filtration and clean-up of solid samples can be achieved as part of the solvent extraction process in a single step. ASE offers a lower cost per sample than other techniques, reducing solvent consumption by up to 90%.


Dionex UltiMate 3000 RSLC Dual




2. Automated On-Line SPE-LC for HPLC Sample Preparation and Automation.

Solid-phase extraction (SPE) is a technique that is commonly used in laboratories to isolate the analytes of interest from complex matrices.

Example of analysis of pork:

Sample preparation: Extract 5,0 g pork chop with approx.. 50 ml of 4:1 acetonitrile : isopropanol using ASE extractor. Chill to precipitate fat, and filter.

In this example, we extracted pork with organic solvents in an Accelerated Solvent Extractor. Evaporation and reconstitution proved troublesome, so we avoided that by using solid-phase extraction (SPE).

SPE is most effective when it is orthogonal to the analytical separation, so we used cation exchange to retain the drugs, and washed away lipids and other interferences with organic solvent. The auxiliary valve then transferred the drugs to an Acclaim C30 column for RP-HPLC analysis.

The leaflet download here.

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  • 31 August 2020
  • Webinar "Nitrogen/Protein Analysis - Dumas method vs Kjeldahl method" Sept. 9, 2020, 11-00 MT

    Abacus and Elementar are invite you to webinar "Nitrogen / Protein Analysis in Foods -
    Dumas method vs Kjeldahl method" September 9, 2020, 11-00 Moscow time

    In this webinar, we will compare the technical characteristics of the Dumas and Kjeldahl methods and discuss their advantages and disadvantages.

    Please be registered in advance:

    Link to webinar registration

    Welcome to our webinar!

    Abacus and Elementar teams.

  • 01 May 2020
  • Webinar "Solving the Problems of Determining the Content of Organic Carbon (TOC) and Nitrogen (TNb) in Wastewater"

    Elementar and Abacus Analytical Systems GmbH invite you to the webinar "Solving the Problems of Determining the Content of Organic Carbon (TOC) and Bound Nitrogen (TNb) in Wastewater", which will be held on May 28, 2020, at 14-00 Moscow time.

  • 31 March 2020
  • With the greatest regret we report the demise of Karl-Heinz Fischer

    Karl-Heinz Fischer


    Karl-Heinz Fischer

    06/18/1937 - 03/31/2020

    Eternal memory to him!

    Elena V. Rybakovaand the team of Abacus GmbH
    in Russia, Kazakhstan, Ukraine, Belarus

  • 01 October 2019
  • The seminar for CHNS-elemental analysis from the company Elementar, 5th December 2019

    "Abacus Analytical Systems" and the Elementar Analysensysteme GmbH (Germany) invite you to participate in a scientific and practical seminar "The latest analytical equipment for CHNS-elemental analysis from the company Elementar". Seminar will take place in Moscow on December 5, 2019 in the conference center at hotel of "Maxima Zarya" (metro Okrujhnaya, Vladykino or Petrovsko- Razumovskaya). Lectures will be given by leading experts in the field of CHNS-elemental analysis from Elementar GmbH, Abacus GmbH and the leading Russian research Institutes.

    Participation in a seminar - free. For participation it is necessary to be registered, write us to the address:

  • 19 May 2019
  • Practical course on ion chromatography 18-22 November 2019 in Moscow

    Nearest practical course on ion chromatography will take place in Moscow on the territory of Thechnical University (MISIS) and MSU (practice) 18-22th November 2019.

    The program of course for an ion chromatography includes the following primary subjects:

        Theoretical bases of an ion chromatography;
        Sorbents for an ion chromatography
        Devices of the ion chromatograph (detectors, design features, etc.);
        Proper laboratory practice (features of selection and preparation of samples for the analysis, methodical recommendations).
        Service of the chromatography equipment

    Please contact us for future details!