How to determine of Ractopamine in meat?
We know and we propose the
Ractopamine is a drug that is used as a feed
additive to promote leanness in animals raised for their meat.
Pharmacologically, it is a beta-adrenoceptor agonist. It is the
active ingredient in products known as Paylean for swine and
Optaflexx for cattle, developed by Elanco Animal Health, a division
of Eli Lilly and Company, for use in food animals for growth
Ractopamine has been banned in the European Union, Taiwan,
mainland China and Russia.
As you know from the news: Rosselkhoznadzor on
February 11, 2013. introduces prohibitions supply frozen meat and
meat products from the United States. This is due to the fact that
the American Veterinary Service does not observe the rules of
ractopamine. Supply of chilled meat for that reason are prohibited
from February 4. The use of ractopamine in Russia is prohibited.
Rosselkhoznadzor notified the case management Safety and Inspection
Service Food USDA.
How to determine of
Ractopamine in meat?
We offer you the solution of this analytical task!
1. Extraction by ASE
The many steps involved in sample preparation can
now be automated with ASE flow-through technology. Filtration and
clean-up of solid samples can be achieved as part of the solvent
extraction process in a single step. ASE offers a lower cost per
sample than other techniques, reducing solvent consumption by up to
2. Automated On-Line SPE-LC for HPLC Sample Preparation
Solid-phase extraction (SPE) is a technique that is commonly
used in laboratories to isolate the analytes of interest from
Example of analysis of pork:
Sample preparation: Extract 5,0 g pork chop with approx.. 50 ml
of 4:1 acetonitrile : isopropanol using ASE extractor. Chill to
precipitate fat, and filter.
In this example, we extracted pork with organic solvents in an
Accelerated Solvent Extractor. Evaporation and reconstitution
proved troublesome, so we avoided that by using solid-phase
SPE is most effective when it is orthogonal to the analytical
separation, so we used cation exchange to retain the drugs, and
washed away lipids and other interferences with organic solvent.
The auxiliary valve then transferred the drugs to an Acclaim C30
column for RP-HPLC analysis.
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